| Notes
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ARA70 has a role in enhancing the androgen receptor during testicular development. ARA70 is a coactivator for estrogen receptor alpha (ERalpha) and may represent a functional link between ERalpha/androgen receptor (AR) modulating their cross-talk in models of estrogen signaling in MCF-7 and HeLa cells. ARA70 was identifed as a coactivator of PPARalpha in the prostate cancer cell line DU145. In the adrenal cell line Y1, ARA70 behaved as a repressor of PPARalpha while still able to coactivate PPARgamma. Domain interactions between coregulator ARA(70) and the androgen receptor (AR); structure activity relationship. Potent mitogenicity of the RET/PTC3 oncogene correlates with its prevalence in tall-cell variant of papillary thyroid carcinoma. The refolding and one-step purification of ARA70 from inclusion bodies over-expressed in Escherichia coli is reported. Together, ARA54 and ARA70 modulate vitamin D receptor transactivation, and the competition for ARA70 mediates the suppressive affect of androgen-androgen receptor on vitamin D receptor transactivation. Causes increases in nuclear NF-kappaB activity and secretion of MCP-1 and GM-CSF; studies implicate oncogene-induced cytokine-signaling pathways in a new mechanism linking inflammation with cancer. Role in reducing agonist activity of antiandrogens.
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